RNA Interference (RNAi) and siRNA Techniques
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Course Overview
Workshop Registration End Date :2025-01-31
Program Syllabus
Module 1
About
RNA interference (RNAi) is a natural cellular process used to regulate gene expression. This 3-day workshop will delve into the fundamental mechanisms behind RNAi and its application in controlling the expression of specific genes. Participants will explore theoretical aspects of small interfering RNA (siRNA) techniques, from the molecular biology involved to real-world applications in research and medicine.
Throughout the workshop, we will focus on the role of RNAi in biological research, including gene function analysis, therapeutic approaches in diseases like cancer and viral infections, and gene editing techniques. The workshop will offer guidance in designing siRNA experiments, selecting appropriate delivery systems, and interpreting results, making it a valuable tool for molecular biologists, geneticists, and medical researchers.
Module 2
Aim
This workshop aims to equip participants with a comprehensive understanding of RNA interference (RNAi) and small interfering RNA (siRNA) techniques. It will cover key mechanisms, applications in gene silencing, and how RNAi can be utilized in research and therapeutic strategies. By the end of the workshop, participants will be able to design and implement RNAi experiments for various biological studies.
Lead Instructor
Dr. Sarah Chen
PhD in Computational Mechanics from MIT with 15+ years of experience in Industrial AI. Former Lead Data Scientist at Tesla and current advisor to Fortune 500 manufacturing firms.
Limited SeatsClosing Soon
RNA Interference (RNAi) and siRNA Techniques
Professional Certification Program
🎥
FormatLive + Recorded
📅
Duration8 Weeks
📜
CertificationVerified
Module 3
Workshop Objectives
- Understand the mechanisms of RNA interference and its biological significance.
- Learn about siRNA design and its applications in gene silencing.
- Explore delivery methods for siRNA in cellular and animal models.
- Analyze case studies of RNAi in therapeutic development.
- Develop skills in interpreting RNAi data and troubleshooting experiments.
Module 4
Workshop Structure
Module 5
Day 2: siRNA Techniques: Design and Delivery
siRNA Basics:
- What is siRNA? Structure and function
- Differences between siRNA and miRNA
- siRNA-mediated gene silencing
Designing siRNAs:
- Key criteria for designing siRNAs
- Tools and software for siRNA design (e.g., Invitrogen BLOCK-iT, siDirect)
- Avoiding off-target effects
- Selection of target sequences, sense and antisense strand considerations
siRNA Delivery Methods:
- Lipid-based delivery systems (Lipofectamine)
- Electroporation and viral vectors (AAV, lentivirus)
- Nanoparticles for siRNA delivery
- Challenges in siRNA delivery: Stability, off-target effects, cellular uptake
siRNA Knockdown Assays:
- Confirming siRNA efficiency
- Techniques: RT-qPCR, Western blotting, and immunofluorescence
Module 6
Day 3: Applications of RNAi and siRNA in Research and Therapeutics
RNAi and Disease Research:
- Role in cancer, neurodegenerative diseases, and viral infections
- Case studies: siRNA therapies in clinical trials (Patisiran for ATTR amyloidosis)
Therapeutic Applications of siRNA:
- RNAi-based drugs: Development and FDA approval (Onpattro, Givlaari)
- Challenges in therapeutic siRNA delivery and stability
- Ongoing clinical trials using RNAi and siRNA
Gene Therapy and siRNA:
- Role of siRNA in gene therapy and personalized medicine
- Ethical considerations and regulatory frameworks
Future Directions in RNAi and siRNA Research:
- Next-generation RNAi technologies
- CRISPR vs RNAi in gene editing
Module 7
Participant’s Eligibility
- Undergraduate or graduate degree in Biotechnology, Molecular Biology, Genetics, Biochemistry, or related fields.
- Professionals working in genetic research, biomedical fields, or pharmaceutical industries.
- Individuals interested in gene therapy, drug development, and molecular diagnostics.
Module 8
Important Dates
Module 9
Registration Ends
2025-01-31
Indian Standard Timing 2:00 PM
Module 10
Workshop Dates
2025-01-31 to 2025-02-02
Indian Standard Timing 3:00 PM
Module 11
Workshop Outcomes
- Proficiency in RNAi mechanisms and siRNA design.
- Knowledge of RNAi-based therapeutic developments.
- Ability to perform gene silencing experiments.
- Enhanced skills in molecular diagnostics using RNA technologies.
Module 12
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Mentor Profile
Dr. Darshan Panda
Scientist
Crop Physiology & Biochemistry, ICAR-NRRI
Dr. Darshan Panda is a Scientist in the Department of Crop Physiology & Biochemistry at the ICAR-National Rice Research Institute, Bidyadharpur, Cuttack. He received his Ph.D. Degree in Biotechnology from Ravenshaw University in 2022. He is working currently as a Scientist at ICAR-NRRI, Cuttack. . . .
Module 13
We are excited to announce that we now accept payments in over 20 global currencies, in addition to USD. Check out our list to see if your preferred currency is supported. Enjoy the convenience and flexibility of paying in your local currency!
List of Currencies
Fee Structure
Module 14
Student
INR. 1399
USD. 50
Module 15
Ph.D. Scholar / Researcher
INR. 1699
USD. 55
Module 16
Academician / Faculty
INR. 2199
USD. 60
Module 17
Industry Professional
INR. 2699
USD. 85
List of Currencies
Module 18
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Key Takeaways
- Access to Live Lectures
- Access to Recorded Sessions
- e-Certificate
- Query Solving Post Workshop
Module 19
Future Career Prospects
- Research Scientist in Molecular Biology
- Biomedical Scientist specializing in Genetic Therapeutics
- Bioinformatics Specialist in Gene Editing
- Pharmacologist in siRNA Drug Development
- Clinical Researcher in Genetic Disorders
- Biotechnologist in RNA-based Therapeutics
Module 20
Job Opportunities
- Pharmaceutical and biotech companies
- Academic and clinical research institutions
- Bioinformatics firms
- Healthcare organizations
- Genetic research
- Gene therapy development
- Molecular diagnostics
- RNA-based drug design
Module 23
Enter the Hall of Fame!
Take your research to the next level!
Publication Opportunity
Potentially earn a place in our coveted Hall of Fame.
Centre of Excellence
Join the esteemed Centre of Excellence.
Networking and Learning
Network with industry leaders, access ongoing learning opportunities.
Hall of Fame
Get your groundbreaking work considered for publication in a prestigious Open Access Journal (worth ₹20,000/USD 1,000).
Achieve excellence and solidify your reputation among the elite!
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Module 24
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