Genome Editing Technologies: Principles and Applications

• Master the foundational principles of genome editing technologies.
• Apply genome editing tools in various biological and medical contexts.
• Evaluate the ethical, legal, and social issues associated with genome editing.
• Develop critical thinking and problem-solving skills in genetic manipulation.
• Prepare for advanced research or career opportunities in genetic technologies.

Week 1: Fundamentals of Genome Editing Technologies

• Introduction to gene structure and regulation

• Evolution of genome editing: ZFNs, TALENs, CRISPR

• Mechanisms of site-specific DNA cleavage and repair

• NHEJ vs HDR: pathway selection and outcomes

Week 2: CRISPR and Beyond – Mechanisms and Toolkits

• CRISPR-Cas9 and its variants (Cas12, Cas13)

• Guide RNA design and PAM sequence specificity

• Base editing and prime editing technologies

• Multiplexing and epigenome editing capabilities

Week 3: Applications in Health, Agriculture, and Research

• Functional genomics and gene knockout models

• Therapeutic genome editing for monogenic diseases

• Agricultural trait enhancement and biotic stress tolerance

• Case studies: Sickle Cell Disease, muscular dystrophy, rice yield improvement

Week 4: Delivery Methods, Ethics, and Future Outlook

• Viral and non-viral delivery strategies (LNPs, electroporation)

• AI and computational tools in target prediction and off-target screening

• Regulatory frameworks and bioethical concerns

• Future prospects: synthetic biology, in vivo editing, germline modifications

Edit

• Comprehensive understanding of genome editing mechanisms and tools
• Ability to design and conduct experiments using genome editing technologies
• Insights into the ethical, social, and regulatory dimensions of genetic editing
• Skills to innovate in fields of genetics and biotechnology
• Preparation for careers or advanced studies in genetic technologies