CRISPR-Cas Genome Editing: Mechanisms and Applications

• Understand the fundamentals of CRISPR-Cas systems and their biological functions.
• Gain hands-on experience with CRISPR design and applications in lab simulations.
• Explore the ethical and regulatory frameworks governing gene editing.
• Analyze case studies on the impact of CRISPR technologies in medicine and agriculture.
• Prepare for advanced studies or careers in genetic editing technologies.

Week 1: CRISPR-Cas Systems – Origins and Molecular Biology

• Discovery of CRISPR in microbial adaptive immunity

• Mechanisms of Cas9, Cas12, and Cas13 nucleases

• Guide RNA design and PAM site recognition

• DNA repair pathways: HDR vs. NHEJ

Week 2: Genome Editing Platforms and Delivery Technologies

• Plasmid, RNP, and viral vector delivery methods

• Off-target effects and specificity optimization

• Deep tech delivery tools: lipid nanoparticles, electroporation

• Multiplex genome editing and gene knock-in strategies

Week 3: CRISPR in Research, Medicine, and Agriculture

• Functional genomics and model organism editing

• Therapeutic applications: cancer, blood disorders, viral diseases

• Crop improvement and gene drive in agriculture

• Real-world case studies and translational breakthroughs

Week 4: Future of CRISPR, Ethics, and Regulation

• Prime editing, base editing, and epigenome modulation

• CRISPR diagnostics (e.g., SHERLOCK, DETECTR platforms)

• Bioethical considerations and global regulatory landscape

• Commercial trends, patent battles, and innovation pipelines

Edit

• Proficiency in CRISPR-Cas technology applications
• Understanding of molecular techniques in genetic engineering
• Ability to address ethical considerations in biotech
• Readiness for advanced research or professional roles
• Skills in interdisciplinary collaboration and innovation