Advanced Digital PCR (dPCR) Multiplexing: Principles, Design, and Applications
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Course Overview
Workshop Registration End Date :09/30/2025
Program Syllabus
Module 1
About
This 3-day hands-on workshop offers an in-depth exploration of multiplex digital PCR (dPCR)—a cutting-edge technique for absolute quantification of nucleic acids. Participants will learn the principles of dPCR, design multiplex assays, troubleshoot experiments, and analyze real-world data using simulation tools. Applications span infectious disease diagnostics, cancer mutation detection, gene expression analysis, and more. Ideal for molecular biologists, diagnostic researchers, and biotech professionals seeking advanced skills in high-precision PCR technologies.
Module 2
Aim
The aim of this workshop is to equip participants with a comprehensive understanding of digital PCR (dPCR) technology, with a special emphasis on multiplex assay design, optimization, and application. Through a combination of theoretical instruction, case-based learning, and hands-on simulation, the workshop intends to enable researchers, clinicians, and molecular biologists to leverage multiplex dPCR for precise, absolute quantification of nucleic acids across a range of fields including infectious disease diagnostics, oncology, genetic mutation detection, and GMO testing.
Module 3
Workshop Objectives
- To introduce the fundamentals of digital PCR and its advantages over qPCR
- To explain the principles and applications of multiplex assay design
- To train participants in primer/probe design and spectral optimization
- To provide hands-on guidance on dPCR instrument operation and data analysis
- To demonstrate the application of dPCR in clinical and research settings
- To enhance skills in interpreting multiplex results and troubleshooting experiments
Module 4
Workshop Structure
Day 1: Fundamentals of dPCR
- Introduction to Digital PCR vs qPCR
- Principles of droplet and chip-based dPCR
- Concept of absolute quantification
- Applications overview (oncology, virology, plant biotech)
Day 2: Multiplexing Design & Optimization
- Primer/probe design strategies
- Avoiding dimer formation and spectral overlap
- Multiplex assay optimization techniques
- Hands-on software simulation: multiplex setup
Day 3: Hands-On dPCR & Data Interpretation
- Running a multiplex dPCR experiment
- Instrument calibration and loading
- Endpoint fluorescence reading and analysis
- Data interpretation, troubleshooting common issues
- Case Study: SARS-CoV-2 and Influenza co-detection
Module 5
Intended For
- Molecular biologists, geneticists, and microbiologists
- Biotech and life science researchers working in diagnostics, oncology, virology, or agriculture
- Postgraduate and PhD students in biotechnology, bioinformatics, or related disciplines
- Clinical lab professionals and diagnostic R&D teams
- Anyone interested in mastering digital PCR and multiplex assay design
Module 6
Important Dates
Module 7
Registration Ends
09/30/2025
Indian Standard Timing 2:00 PM
Module 8
Workshop Dates
09/30/2025 to 10/02/2025
Indian Standard Timing 3:00 PM
Module 9
Workshop Outcomes
- Understand the principles and workflow of digital PCR and multiplexing
- Be able to design and optimize multiplex dPCR assays
- Gain hands-on experience with dPCR setup, calibration, and data analysis
- Learn to troubleshoot common issues in multiplex assays
- Apply dPCR knowledge to real-world cases in infectious disease, oncology, and GMO detection
- Receive a certificate of participation validating their practical and theoretical competence
Module 10
We are excited to announce that we now accept payments in over 20 global currencies, in addition to USD. Check out our list to see if your preferred currency is supported. Enjoy the convenience and flexibility of paying in your local currency!
List of Currencies
Fee Structure
Module 14
Industry Professional Fee
INR. 2699
USD. 80
List of Currencies
Module 15
FOR QUERIES, FEEDBACK OR ASSISTANCE
Module 16
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Key Takeaways
- Access to Live Lectures
- Access to Recorded Sessions
- e-Certificate
- Query Solving Post Workshop
Module 17
Future Career Prospects
- Expansion of dPCR in clinical diagnostics, enabling ultra-sensitive detection of infectious agents, cancer biomarkers, and rare mutations
- Integration of dPCR with automation and AI for high-throughput precision testing and data-driven diagnostics
- Development of portable dPCR platforms for field applications in agriculture, environmental monitoring, and point-of-care testing
- Enhanced multiplexing capabilities to allow simultaneous detection of multiple targets, improving cost-efficiency and diagnostic turnaround
- Growing role in regulatory and compliance testing, such as GMO detection and food safety
- Emerging applications in gene therapy monitoring, liquid biopsy, and personalized medicine
Module 18
Job Opportunities
- Molecular Diagnostics Specialist
- PCR Assay Development Scientist
- Clinical Research Associate (CRA)
- Genomics/Biotech Research Scientist
- Molecular Lab Technologist
- Bioinformatics Analyst (PCR Data)
- Quality Control/Validation Scientist in biotech and diagnostic labs
- R&D Scientist in pharmaceuticals, agritech, and clinical genomics
- Regulatory Affairs Associate for molecular testing compliance
Module 21
Enter the Hall of Fame!
Take your research to the next level!
Publication Opportunity
Potentially earn a place in our coveted Hall of Fame.
Centre of Excellence
Join the esteemed Centre of Excellence.
Networking and Learning
Network with industry leaders, access ongoing learning opportunities.
Hall of Fame
Get your groundbreaking work considered for publication in a prestigious Open Access Journal (worth ₹20,000/USD 1,000).
Achieve excellence and solidify your reputation among the elite!
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Module 22
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Lead Instructor
Dr. Sarah Chen
PhD in Computational Mechanics from MIT with 15+ years of experience in Industrial AI. Former Lead Data Scientist at Tesla and current advisor to Fortune 500 manufacturing firms.
Limited SeatsClosing Soon
Advanced Digital PCR (dPCR) Multiplexing: Principles, Design, and Applications
Professional Certification Program
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FormatLive + Recorded
📅
Duration8 Weeks
📜
CertificationVerified
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